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51.
Morelli F Peluso G Petillo O Giannattasio A Filosa S Motta CM Tammaro S Zatterale A Calzone R Budillon A Cartenì M de Maria S Costanza MR Nigro A Petrazzuolo M Buommino E Rizzo M Capasso G Baiano S Moscatiello F Ravagnan G Fuggetta MP Tajana G Stiuso P Metafora BM Metafora V Metafora S 《Journal of cellular physiology》2007,212(3):610-625
Serum deprivation induced in human lymphoblastoid Raji cells oxidative stress-associated apoptotic death and G0/G1 cell cycle arrest. Addition into culture medium of the immunomodulatory protein Seminal vesicle protein 4 (SV-IV) protected these cells against apoptosis but not against cycle arrest. The antiapoptotic activity was related to: (1) decrease of endocellular reactive Oxygen species (ROS) (2) increase of mRNAs encoding anti-oxidant enzymes (catalase, G6PD) and antiapoptotic proteins (survivin, cox-1, Hsp70, c-Fos); (3) decrease of mRNAs encoding proapoptotic proteins (c-myc, Bax, caspase-3, Apaf-1). The biochemical changes underlaying these effects were probably induced by a protein tyrosine kinase (PTK) activity triggered by the binding of SV-IV to its putative plasma membrane receptors. The ineffectiveness of SV-IV to abrogate the cycle arrest was accounted for by its downregulating effects on D1,3/E G1-cyclins and CdK2/4 gene expression, ppRb/pRb ratio, and intracellular ROS concentration. In conclusion, these experiments: (1) prove that SV-IV acts as a cell survival factor; (2) suggest the involvement of a PTK in SV-IV signaling; (3) point to cell cycle-linked enzyme inhibition as responsible for cycle arrest; (4) provide a model to dissect the cycle arrest and apoptosis induced by serum withdrawal; (5) imply a possible role of SV-IV in the survival of hemiallogenic implanting embryos. 相似文献
52.
53.
Borsboom GJ Boatin BA Nagelkerke NJ Agoua H Akpoboua KL Alley EW Bissan Y Renz A Yameogo L Remme JH Habbema JD 《Filaria journal》2003,2(1):8-25
BACKGROUND: The Onchocerciasis Control Program (OCP) in West Africa has been closed down at the end of 2002. All subsequent control will be transferred to the participating countries and will almost entirely be based on periodic mass treatment with ivermectin. This makes the question whether elimination of infection or eradication of onchocerciasis can be achieved using this strategy of critical importance. This study was undertaken to explore this issue. METHODS: An empirical approach was adopted in which a comprehensive analysis was undertaken of available data on the impact of more than a decade of ivermectin treatment on onchocerciasis infection and transmission. Relevant entomological and epidemiological data from 14 river basins in the OCP and one basin in Cameroon were reviewed. Areas were distinguished by frequency of treatment (6-monthly or annually), endemicity level and additional control measures such as vector control. Assessment of results were in terms of epidemiological and entomological parameters, and as a measure of inputs, therapeutic and geographical coverage rates were used. RESULTS: In all of the river basins studied, ivermectin treatment sharply reduced prevalence and intensity of infection. Significant transmission, however, is still ongoing in some basins after 10-12 years of ivermectin treatment. In other basins, transmission may have been interrupted, but this needs to be confirmed by in-depth evaluations. In one mesoendemic basin, where 20 rounds of four-monthly treatment reduced prevalence of infection to levels as low as 2-3%, there was significant recrudescence of infection within a few years after interruption of treatment. CONCLUSIONS: Ivermectin treatment has been very successful in eliminating onchocerciasis as a public health problem. However, the results presented in this paper make it almost certain that repeated ivermectin mass treatment will not lead to the elimination of transmission of onchocerciasis from West Africa. Data on 6-monthly treatments are not sufficient to draw definitive conclusions. 相似文献
54.
Testing lack of fit in multiple regression 总被引:2,自引:0,他引:2
55.
Schiraldi C Martino A Acone M Di Lernia I Di Lazzaro A Marulli F Generoso M Cartenì M De Rosa M 《Biotechnology and bioengineering》2000,70(6):670-676
A microfiltration (MF) membrane bioreactor was developed for an efficient production of a recombinant thermostable alpha-glucosidase (rSsGA) from Sulfolobus solfataricus MT-4. The aim of the membrane bioreactor was to improve the control of the concentration of key components in the growth of genetic engineered microorganisms, such as Escherichia coli. The influence of medium composition was studied in relation to cell growth and alpha-glucosidase production. The addition of components such as yeast extract and tryptone resulted in a higher enzyme production. High cell density cultivation of E. coli BL21(DE3) on semidefined medium, exploiting a microfiltration bioreactor, was studied in order to optimize rSsGA production. In addition to medium composition, the inducer employed (either isopropyl beta-D-thiogalactopyranoside or lactose), the induction duration, and the cultivation mode influenced both the final biomass and the enzyme yield. The MF bioreactor allowed a cell concentration of 50 g/L dry weight and a corresponding alpha-glucosidase production of 11,500 U/L. The improvement obtained in the enzyme production combining genetic engineering and the microfiltration strategy was estimated to be 2,000-fold the wild-type strain. 相似文献
56.
An anchored restriction-mapping approach applied to the genetic analysis of the Anopheles gambiae malaria vector complex 1 总被引:1,自引:0,他引:1
We introduce here a simple approach for rapidly determining restriction
maps for a number of regions of a genome; this involves "anchoring" a map
with a rare restriction site (in this case the seldom-cutting EagI)
followed by partial digestion of a frequent-cutting enzyme (e.g., Sau 3A).
We applied this technology to five species of the Anopheles gambiae
complex. In a single Southern blot we obtained about a 15-kb restriction
map each for the mtDNA, rRNA gene, and a scnDNA region for each of five
species. Phylogenetic analyses of these regions yield trees at odds with
the more traditional chromosome inversion-based trees. The value of the
approach for systematic purposes is the ease with which several large,
independent regions of the genome can be quickly assayed for molecular
variation.
相似文献
57.
58.
Pontoni G Rotondo F Spagnuolo G Aurino MT Cartenì-Farina M Zappia V Lama G 《Amino acids》2000,19(2):469-476
Summary. Proton Nuclear Magnetic Resonance (NMR) Spectroscopy of urine (as well as of other biological fluids) is a very powerful
technique enabling multi-component analysis useful in both diagnosis and follow-up of a wide range of inherited metabolic
diseases. Among these pathologies, cystinuria is characterised by accumulation in urine of four dibasic amino acids, namely
lysine, arginine, ornithine and cystine; the last one, being only slightly water soluble, generates urolithiasis. The mentioned
aminoacids can be detected in the urine NMR spectrum of cystinuric patients, the most abundant being the lysine (5 mM and
over are often detected), whose typical signals become very high; arginine and ornithine are also usually detectable, although
pathologic concentrations are lower (usually below 2 mM).
The proposed NMR technique is also suitable in monitoring the therapy with α-mercaptopropionylglycine (MPG), providing quantitation
of several metabolites of interest in the follow-up of the pathology, like cystine, creatinine and citrate.
Received May 9, 1999; Accepted September 26, 1999 相似文献
59.
K Kertesz-Chaloupková PJ Walser JD Granado M Aebi U Kües 《Fungal genetics and biology : FG & B》1998,23(1):95-109
Monokaryotic mycelia of the homobasidiomycete Coprinus cinereus form asexual spores (oidia) constitutively in abundant numbers. Mycelia with mutations in both mating type loci (Amut Bmut homokaryons) also produce copious oidia but only when exposed to blue light. We used such an Amut Bmut homokaryon to define environmental and inherent factors that influence the light-induced oidiation process. We show that the Amut function causes repression of oidiation in the dark and that light overrides this effect. Similarly, compatible genes from different haplotypes of the A mating type locus repress sporulation in the dark and not in the light. Compatible products of the B mating type locus reduce the outcome of light on A-mediated repression but the mutated B function present in the Amut Bmut homokaryons is not effective. In dikaryons, the coordinated regulation of asexual sporulation by compatible A and B mating type genes results in moderate oidia production in light. Copyright 1998 Academic Press. 相似文献
60.
Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3 总被引:1,自引:0,他引:1
Cornillot JD; Pontet M; Dupuy C; Chadli A; Caron M; Joubert-Caron R; Bourin P; Bladier D 《Glycobiology》1998,8(5):425-432
Antisera raised against galectin-1 exhibit crossreactivities with other
galectins or related molecules. In order to overcome this problem, a
monoclonal antibody to human brain galectin-1 was obtained by selecting
clones without reactivity toward galectin-3. This mAb specifically bound
galectin-1 of various animal origins but neither galectin-2 nor galectin-3.
Western-blotting analysis of soluble human brain extracts after 2D gel
electrophoresis revealed only the two most acidic isoforms of galectin-1.
The ability of this mAb to bind galectin-1/asialofetuin complexes indicates
that its epitope is not localized in the carbohydrate recognition domain of
galectin-1. This particularity induces with efficiency its monospecificity.
相似文献